Pollen – INSIGNIA need pollen!

This spring is different from the last years. Seems that spring is coming earlier an earlier. We had a warm early period. This year everting started 2 weeks earlier. Beekeepers were of course concerned. Will the bees become ready? And yes, colonies looked very nice and winter losses seems to be low. The rape fields are ready now. Dandelion all over. And then we got frost during night time. Not good for the apples! Cold wind from the east. Bees stopped flying! But as always, there are some moments every day, where the bees do fly and collect pollen. Continue reading “Pollen – INSIGNIA need pollen!”

Denmark right now


Denmark is a heavy farming country. More than 60% of the surface is cultured landscape by farmers. In May the country is more or less yellow. You will see it when flying over the country. This year we have around 160.000 hectare of rape fields. Rape is a very good nectar and pollen flower give the main flow in Denmark. Rape honey is a very light and creamy honey. Danish customers love this honey. On the other side, it is a very intensive crop. From the putting the seed into the soil to the harvest, on average the rape culture is sprayed up to 6 times with pesticides.
Flemming Vejsnæs

 

How to store pollen for analysis

Pollen sampling by citizen scientists (CS) is underway in several countries. How to store the pollen until molecular analysis? How to transport the pollen between the CS and the lab? These are important questions addressed by INSIGNIA. Storage and transportation will influence DNA quality and quantity, both important features for reliable botanical identification via metabarcoding. INSIGNIA compared storage in ethanol with freezing. It turns out that storage in ethanol assures good DNA quality quantity, which makes storage and transportation easier.
Alice Pinto

Collecting beebread

The first official sampling period is right around the corner, so it is time to talk about the sampling procedures in detail. Citizen scientists sample beebread from colony 1 and colony 3. Originally, the idea was to use a specially developed and homemade so-called “beebread collector” to collect the beebread. Nevertheless, in the last few weeks we concluded that simple straws cut in pieces are a better solution. They are even cheaper than the BB-collector, disposable (no exchange of diseases or pesticides) and easier in handling. The aim is to sample in total 3 g of beebread per colony, which is equivalent to 60 cells or about 5 cm of beebread in the straws.

Process of shortening the straws to 2-3 cm pieces

Continue reading “Collecting beebread”

Citizen Science – innovation at its best

Some blog entries ago we introduced an elegant way of holding the Beehold tubes in colony 3 by inserting 4 holes into a sliding gate. This innovative solution was based on the idea of one of our citizen scientists from Austria. As mentioned in the previous blog posting, bees of colony 1 had or still have issues to get used to the experimental setup but for our citizen scientists this is no reason to throw in the towel. Instead, one of our participants (clever, Roland!) invented a nice, cheap and easy solution to improve the adaption of bees to the new circumstances: firstly, he mounted a landing board to facilitate the traffic of bees; secondly, he installed a mechanism to open and close the pollen trap without disturbing them even if the adapter for Beehold tubes is mounted. Now, that is the magic of citizen science.

Modified hive entrance for INSIGNIA monitoring colony 1. A DIY mechanism to (de-)activate the pollen trap from outside and a landing board to ease the access of bees.
Scheme of the DIY mechanism designed by an Austrian citizen scientist.

Colony 1 – Bees not finding their way easily

Reminder: in colony 1 all of the four sampling matrixes are tested. Thus, bees not only have to get used to the pollen traps but also to the Beehold tubes and the corresponding adapter. This might be “overwhelming” for some of the colonies and in a consequence, several messages from worried citizen scientists arrived us during the last week. They all reported similar problems, observed in colony 1: bees have issues to find the right path into their colonies and they congregate in front of the hive entrance; bees show increased aggressive behavior; bees use the drone entrance to get into the hive and some of them were also observed to fly into the neighboring colonies.

Bees cluster in front of the hive entrance

Continue reading “Colony 1 – Bees not finding their way easily”

The very first sampling

Even the first pollen samples and beebread samples will be collected in 1 weeks’ time, we did test the different methods in Denmark already. Just to see.

We need from each colony 30 gram of pollen. No problem.

We need 3 grams of beebread collected via straws. This means that we need to use 60 cells in the colony. This is the most time-consuming part of the sampling.

Introducing the Apistrips and the Beeholdtubes are very easy. No problems for the bees, but as usual, they need to get use to the new situation, but after some moments, everything is like it use to be.

We are looking forward to next weeks “real” sampling.
Flemming Vejsnæs

 

The 4 apiaries in Denmark

In Denmark we have 4 apiaries. We are lucky that we have a pollen collecting group, all using the same bottom board for pollen collection. That make the test setup a bit easier.
Apiary no 1. This is the beekeeper’s association’s apiary. Colonies do belong to the association, but the colonies are run by a private beekeeper. This apiary is just beside one of our very heavy motorways. Industrial area and medium intensive farming. Polluted area.
Apiary no. 2. At a beekeeper’s place. Medium intensive farming area. Relative intense pig production. Moor area, one of the biggest in Denmark, but still with farming. Polluted area.
Apiary no. 3. Is at a beekeeper’s summerhouse. Big forest area. If we would move the colonies just 1 km it would be an organic apiary. No pollution.
Apiary no. 4. Was mentioned in an earlier blog. At an organic beekeeper’s place and therefore expected no pollution.

 

A Danish Citizen Scientist

Peter Sjøgren is one of the Danish Citizen Scientist. We did approach Peter since we needed 2 apiaries in you could say more polluted areas and 2 in less polluted areas within the INSIGNIA project. Denmark is a small country with intense farming. Peter is always open minded and open to give his share for improving beekeeping. Peter is one of the very few Danish organic beekeepers. Living north of Copenhagen, where we have the very big forest area of Gribskov. He is just on the corner of the area where it is possibly to become approved as organic beekeeper. We regard this area as a low pollution area. Peter is one of our very innovative beekeepers. Always on the move for explore new within beekeeping. Take a look on his personal homepage: www.honningpresse.dk 

Greek INSIGNIA tool box ready

Hello all,

Our tool boxes are ready to be posted  to Citizen Scientists! They have everything, at least for the start. Only towel paper is missing! Note that 2 pollen traps are also included. Unfortunately a third pollen trap did not fit in the box!

Lets start then!

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